Characterization of postnatal inducible whole-body Ngly1 knockout mice. (A) Schematic diagram showing the generation of postnatal inducible whole-body Ngly1 knockout (iNgly1^−/−) mice. (B) Western blot analysis of the NGLY1 protein in brain, liver, and spleen of iNgly1^−/− and Ngly1^fl/fl control mice. The data were verified in at least two independent experiments. (C) Body weight of iNgly1^−/− and Ngly1^fl/fl mice (n = 4–25 each time point). Data are shown as the mean ± SEM. Two-way ANOVA. ***P < 0.001. (D) Survival curves of iNgly1^−/− (n = 49 males, 40 females) and Ngly1^fl/fl (n = 38 males, 45 females) mice. Log-rank (Mantel–Cox) test. **P < 0.01; ***P < 0.001. (E) Representative image of kyphosis of iNgly1^−/− mice at 3 mo old. (F) Neurological deficit score of iNgly1^−/− and Ngly1^fl/fl mice. Data are shown as median ± 95% CI. Mann–Whitney U test. ***P < 0.001. Source data are available for this figure: SourceData F1.