N- and C-terminal peptides bind HLA class I alleles promiscuously. (A) Peptide-binding prediction of PfCSP peptides to HLA-A and HLA-B alleles of five vaccinated donors by NetMHCpan4.1 tool. Predicted peptides (9–11mer length, EL-rank threshold below 7) within the FMP013 aa sequence (position 20–380) are highlighted in color and shown within starting position ranges of 10 aa. (B and C) Binding of seven predicted PfCSP peptides to five HLA monomer. (B) FACS plot of β-microglobulin–stained monomers loaded with individual peptides compared with allele-specific negative and positive controls. (C) Fold change in MFI of peptide-loaded monomers relative to allele-specific negative controls. (B and C) represent one experiment. MFI, median fluorescence intensity.