Myo15 localizes to the presynaptic bouton. (A) Confocal images of control and Myo15-RNAi knock-down NMJs stained for Myo15 (anti-Myo15C1, green) and HRP (magenta), scale bar 5 µm. (B) Quantification of A. Myo15C1 mean pixel intensity (control 100.0 ± 6.89%, n = 12; Myo15-RNAi 35.08 ± 3.0%, n = 12). (C) Western blot of wild-type and myo15−/− mutant larva for Myo15C1. (D) Confocal images of NMJs upon motoneuronal Myo15 overexpression and control, stained for Myo15C1 (green) and HRP (magenta), scale bar 5 µm. (E) Quantification of D. Myo15C1 mean pixel intensity (control 100.0 ± 11.99%, n = 11; Myo15 OE 207.6 ± 25.55%, n = 12). (F) Western blot of adult brains neuronally overexpressing Myo15 and control brains for Myo15C1. (G) Confocal image of wild-type NMJ stained for Myo15 (anti-Myo15C1, green) and BRP (magenta), scale bar overview 5 µm, zoom 2 µm. White arrowheads mark Myo15 accumulations near or at an AZ, yellow arrowheads mark AZ without Myo15 accumulations. (H) STED imaging of wild-type NMJs stained for Myo15 (anti-Myo15C1, green) and BRP (magenta), scale bar overview 1 µm, zoom 0.1 µm. White square marks the zoom area, the white dashed line in the zoom marks the plane of line profile. Lower panel shows line profile, arrowheads as in G. All data are provided as mean ± SEM. (B and E)n represents NMJs with 1–2 NMJs/animal from five to six animals, ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05; n.s., not significant. OE, overexpression. Source data are available for this figure: SourceData S4.
Figure 4.

Myo15 localizes to the presynaptic bouton. (A) Confocal images of control and Myo15-RNAi knock-down NMJs stained for Myo15 (anti-Myo15C1, green) and HRP (magenta), scale bar 5 µm. (B) Quantification of A. Myo15C1 mean pixel intensity (control 100.0 ± 6.89%, n = 12; Myo15-RNAi 35.08 ± 3.0%, n = 12). (C) Western blot of wild-type and myo15−/− mutant larva for Myo15C1. (D) Confocal images of NMJs upon motoneuronal Myo15 overexpression and control, stained for Myo15C1 (green) and HRP (magenta), scale bar 5 µm. (E) Quantification of D. Myo15C1 mean pixel intensity (control 100.0 ± 11.99%, n = 11; Myo15 OE 207.6 ± 25.55%, n = 12). (F) Western blot of adult brains neuronally overexpressing Myo15 and control brains for Myo15C1. (G) Confocal image of wild-type NMJ stained for Myo15 (anti-Myo15C1, green) and BRP (magenta), scale bar overview 5 µm, zoom 2 µm. White arrowheads mark Myo15 accumulations near or at an AZ, yellow arrowheads mark AZ without Myo15 accumulations. (H) STED imaging of wild-type NMJs stained for Myo15 (anti-Myo15C1, green) and BRP (magenta), scale bar overview 1 µm, zoom 0.1 µm. White square marks the zoom area, the white dashed line in the zoom marks the plane of line profile. Lower panel shows line profile, arrowheads as in G. All data are provided as mean ± SEM. (B and E)n represents NMJs with 1–2 NMJs/animal from five to six animals, ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05; n.s., not significant. OE, overexpression. Source data are available for this figure: SourceData S4.

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