Figure 6.
Effects of vimentin repositioning on cell stiffness. (A and B) Schematic depiction of a control and a rapalog-treated U2OS cell co-transfected with Vim-mCh-FKBP and FRB-BFP-GCN4-ppKin14, before and during indentation in the perinuclear region using a spherical tip of 3.5-µm radius. Each cell was indented at three separate locations in the perinuclear region. (C) The graph presents the Young’s modulus of cells co-transfected with Vim-mCh-FKBP and FRB-BFP-GCN4-ppKin14 constructs, with and without rapalog treatment. Data were collected from n = 19–22 cells across two independent experiments. A total of 56 measurements were obtained from 19 cells without rapalog, and 63 measurements from 22 cells treated with rapalog. The plots display the mean ± SD, with each dot representing an individual cell measurement. ****, P < 0.0001 via Mann–Whitney test.

Effects of vimentin repositioning on cell stiffness. (A and B) Schematic depiction of a control and a rapalog-treated U2OS cell co-transfected with Vim-mCh-FKBP and FRB-BFP-GCN4-ppKin14, before and during indentation in the perinuclear region using a spherical tip of 3.5-µm radius. Each cell was indented at three separate locations in the perinuclear region. (C) The graph presents the Young’s modulus of cells co-transfected with Vim-mCh-FKBP and FRB-BFP-GCN4-ppKin14 constructs, with and without rapalog treatment. Data were collected from n = 19–22 cells across two independent experiments. A total of 56 measurements were obtained from 19 cells without rapalog, and 63 measurements from 22 cells treated with rapalog. The plots display the mean ± SD, with each dot representing an individual cell measurement. ****, P < 0.0001 via Mann–Whitney test.

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