Endogenous vimentin and keratin-8 filament networks in COS-7, U2OS, and HeLa cells. (A) Expression levels of vimentin and keratin-8 in COS-7, U2OS, and HeLa cells were analyzed by western blotting. (B–G) Airyscan high-resolution images of vimentin and keratin-8 networks in COS-7 (B), U2OS (D), and HeLa cells (F), detected by staining with anti-vimentin and anti–keratin-8 antibodies, respectively. Zoomed-in views (5 × 5 µm), highlighted by the dashed box, showing the region of the cell enlarged in the zoom panel and intensity profiles for vimentin and keratin-8 along the indicated lines in COS-7 (C), U2OS (E), and HeLa (G) cells. Source data are available for this figure: SourceData FS4.