KIF5A overexpression diminishes the abundance of acetylated microtubules. (A) Representative images of U2OS cells, either untransfected or transfected with HA-KIF5A-FRB, stained for acetylated microtubules (Ac-tubulin). The transfected cell is outlined with a dashed line. (B) Quantification of normalized acetylated microtubule intensity in untransfected U2OS cells and HA-KIF5A-FRB–expressing cells. A total of 31–32 cells were analyzed across three independent experiments. Data are presented as mean ± SD, with individual cell measurements shown as dots. ****, P < 0.0001 based on Mann–Whitney statistical analysis.