Figure 3.
Quantification of cMyBP-C phosphorylation after PKA treatment of porcine cardiac myofibrils. Protein was isolated from PKA-treated myofibrils and compared with untreated myofibrils. (A) Samples were run on a 4–20% gradient SDS-PAGE gel and stained with Pro-Q Diamond Phosphoprotein Gel Stain (right) followed by Coomassie blue stain (left) to quantify total protein. (B) Densitometry analysis was performed on the cMyBP-C Pro-Q bands normalized to the same band in the Coomassie gel, and cMyBP-C treatment with PKA results in a ∼2.5-fold increase in phosphorylation relative to the untreated group (N = 1, n = 2). Data shown are the mean ± SEM. Significance was calculated with a one-tailed t test, *P = 0.025. Source data are available for this figure: SourceData F3.

Quantification of cMyBP-C phosphorylation after PKA treatment of porcine cardiac myofibrils. Protein was isolated from PKA-treated myofibrils and compared with untreated myofibrils. (A) Samples were run on a 4–20% gradient SDS-PAGE gel and stained with Pro-Q Diamond Phosphoprotein Gel Stain (right) followed by Coomassie blue stain (left) to quantify total protein. (B) Densitometry analysis was performed on the cMyBP-C Pro-Q bands normalized to the same band in the Coomassie gel, and cMyBP-C treatment with PKA results in a ∼2.5-fold increase in phosphorylation relative to the untreated group (N = 1, n = 2). Data shown are the mean ± SEM. Significance was calculated with a one-tailed t test, *P = 0.025. Source data are available for this figure: SourceData F3.

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