Phosphoproteomic changes between meiosis and mitosis and upon CK2 inhibition. (A–C) Volcano plots of dataset 1 log₂ ratio (phosphopeptide abundance) versus −log₁₀ P value for individual phosphopeptides in meiosis compared to mitosis (ME/MI) (n = 14,721 phosphopeptides) (A), meiosis in the presence of CK2i compared to meiosis in the absence of CK2i (n = 14,721 phosphopeptides) (B), and mitosis in the presence of CK2i compared to mitosis in the absence of CK2i (n = 14,721 phosphopeptides) (C), respectively. Red points indicate significantly enriched (log₂ ratio >1 and P value <0.05) phosphopeptides (total number indicated in red). Blue points indicate significantly depleted (log₂ ratio less than −1 and P value <0.05) phosphopeptides (total number indicated in blue). (D) Venn diagram indicating the number of unique phosphopeptides and proteins containing phosphopeptides in dataset 1 in the categories enriched in meiosis relative to mitosis and enriched in meiosis in the absence of CK2i relative to meiosis in the presence of CK2i and in the overlap between these two categories that do not exhibit protein-level changes in the same directions. (E) Venn diagram indicating the number of unique phosphopepetides and proteins containing phosphopeptides in dataset 1 in the categories enriched in mitosis relative to meiosis and enriched in meiosis in the presence of CK2i relative to meiosis in the absence of CK2i and in the overlap between these two categories that do not exhibit protein-level changes in the same directions. (F–H) Volcano plots of dataset 2 log₂ ratio (phosphopeptide abundance) versus −log₁₀ P value for individual phosphopeptides in meiosis compared to mitosis (ME/MI) (n = 13,892 phosphopeptides) (F), meiosis in the presence of CK2i compared to meiosis in the absence of CK2i (n = 13,863 phosphopeptides) (G), and mitosis in the presence of CK2i compared to mitosis in the absence of CK2i (n = 13,877 phosphopeptides), (H) respectively. Red points indicate significantly enriched (log₂ ratio >1 and P value <0.05) phosphopeptides (total number indicated in red). Blue points indicate significantly depleted (log₂ ratio less than −1 and P value <0.05) phosphopeptides (total number indicated in blue). n = 13,892 phosphopeptides. (I) Venn diagram indicating the number of unique phosphopeptides and proteins containing phosphopeptides in dataset 2 in the categories enriched in meiosis relative to mitosis and enriched in meiosis in the absence of CK2i relative to meiosis in the presence of CK2i and in the overlap between these two categories that do not exhibit protein-level changes in the same directions. (J) Venn diagram indicating the number of unique phosphopepetides and proteins containing phosphopeptides in dataset 2 in the categories enriched in mitosis relative to meiosis and enriched in meiosis in the presence of CK2i relative to meiosis in the absence of CK2i and in the overlap between these two categories that do not exhibit protein-level changes in the same directions.