Proteomic changes between meiosis and mitosis and upon CK2 inhibition. (A–C) Volcano plots of dataset 1 log₂ ratio (protein abundance) versus −log₁₀ P value for individual proteins in meiosis compared to mitosis (ME/MI) (A), meiosis in the presence of CK2i compared to meiosis in the absence of CK2i (B), and mitosis in the presence of CK2i compared to mitosis in the absence of CK2i (C), respectively. Red points indicate significantly enriched (log₂ ratio >1 and P value <0.05) proteins (total number indicated in red). Blue points indicate significantly depleted (log₂ ratio less than −1 and P value <0.05) proteins (total number indicated in blue). n = 8,157 proteins. (D) Venn diagram indicating the number of unique proteins in dataset 1 in the categories enriched in meiosis relative to mitosis and enriched in meiosis in the absence of CK2i relative to meiosis in the presence of CK2i and in the overlap between these two categories. (E) Venn diagram indicating the number of unique proteins in dataset 1 in the categories enriched in mitosis relative to meiosis and enriched in meiosis in the presence of CK2i relative to meiosis in the absence of CK2i and in the overlap between these two categories. (F–H) Volcano plots of dataset 2 log₂ ratio (protein abundance) versus −log₁₀ P value for individual proteins in meiosis compared to mitosis (ME/MI) (F), meiosis in the presence of CK2i compared to meiosis in the absence of CK2i (G), and mitosis in the presence of CK2i compared to mitosis in the absence of CK2i (H), respectively. Red points indicate significantly enriched (log₂ ratio >1 and P value <0.05) proteins (total number indicated in red). Blue points indicate significantly depleted (log₂ ratio less than −1 and P value <0.05) proteins (total number indicated in blue). n = 7,948 proteins. (I) Venn diagram indicating the number of unique proteins in dataset 2 in the categories enriched in meiosis relative to mitosis and enriched in meiosis in the absence of CK2i relative to meiosis in the presence of CK2i and in the overlap between these two categories. (J) Venn diagram indicating the number of unique proteins in dataset 2 in the categories enriched in mitosis relative to meiosis and enriched in meiosis in the presence of CK2i relative to meiosis in the absence of CK2i and in the overlap between these two categories.