Proteomic and phosphoproteomic changes between meiosis and mitosis. (A) Schematic of phosphoproteomics experimental setup. Xenopus illustrations © Natalya Zahn (2022) (Fisher et al., 2023; Zahn et al., 2022). (B) Volcano plot of log₂ ratio (protein abundance) versus −log₁₀ P value for individual proteins in meiosis compared to mitosis (ME/MI). Red points indicate significantly enriched (log₂ ratio >1 and P value <0.05) proteins (total number indicated in red). Blue points indicate significantly depleted (log₂ ratio less than −1 and P value <0.05) proteins (total number indicated in blue). n = 16,105 proteins. (C) Volcano plot of log₂ ratio (phosphopeptide abundance) versus −log₁₀ P value for individual phosphopeptides in meiosis compared to mitosis (ME/MI). Red points indicate significantly enriched (log₂ ratio >1 and P value <0.05) phosphopeptides (total number indicated in red). Blue points indicate significantly depleted (ratio log₂ less than −1 and P value <0.05) phosphopeptides (total number indicated in blue). n = 28,612 phosphopeptides. Datasets 1 and 2 were combined and visualized together in this figure. Individual datasets are visualized separately in Fig. S4 (proteome) and Fig. S5 (phosphoproteome).