Super-resolution microscopy of biological samples. (A) Conventional wide-field image (left) and 3D-SIM image of a mouse C2C12 prometaphase cell stained with primary antibodies against lamin B and tubulin, and secondary antibodies conjugated to Alexa 488 (green) and Alexa 594 (red), respectively. Nuclear chromatin was stained with DAPI (blue). 3D image stacks were acquired with a DeltaVision OMX prototype system (Applied Precision). The bottom panel shows the respective orthogonal cross sections. (B) HeLa cell stained with primary antibodies against the nuclear pore complex protein Nup153 and secondary antibodies conjugated with ATTO647N. The image was acquired with a TCS STED confocal microscope (Leica). (C) TdEosFP-paxillin expressed in a Hep G2 cell to label adhesion complexes at the lower surface. The image was acquired on an ELYRA P.1 prototype system (Carl Zeiss, Inc.) using TIRF illumination. Single molecule positional information was projected from 10,000 frames recorded at 30 frames per second. On the left, signals were summed up to generate a TIRF image with conventional wide-field lateral resolution. Bars: 5 µm (insets, 0.5 µm).