Blood indices in Fanca ^−/− , Aldh9a1 ^−/− , and dKO mice and gene expression of ALDH and ADH genes in humans and mice. (A) Mouse HSC flow gating scheme used for Fig. 4, D–H and Fig. S2 B. (B) Frequency of lineage-negative/cKit-high population, MPP, CMP, and GMP of live single cells from fresh bone marrow aspirate samples. Number of mice analyzed: WT n = 14, Aldh9a1 KO n = 12, Fanca KO n = 13, and dKO n = 13; 8–12 wk old. One-way ANOVA followed by Tukey’s multiple comparison test was performed (ns, not significant; *, P < 0.05). Error bars, SD. MPP, multipotent progenitor; CMP, common myeloid progenitor; GMP, granulocyte-macrophage progenitor. (C) Peripheral blood platelet counts of 9- to 12-mo-old mice (WT n = 9, Aldh9a1 KO n = 44, Fanca KO n = 9, and dKO n = 45). One-way ANOVA followed by Tukey’s multiple comparison test (**, P = 0.0012). Error bars, SD. (D) Gene expression data for human and mouse ALDH and ADH isoenzymes in hematopoietic cells. Publicly available single-cell RNA-seq data were downloaded from BloodSpot.eu and a heatmap was generated using log₂ fold change values on GraphPad Prism v10. Mouse hematopoietic cells show a predominant ALDH2 expression, while human hematopoietic cells show higher expression of other ALDH enzymes. dKO, double KO.