Loss of ICAM-1 reduces tissue ILC2s. (A) Representative flow cytometry plots of ILC2s in the peripheral tissues from WT and ICAM-1^−/− mice, including lung (CD45⁺Lin⁻CD90.2⁺CD25⁺), mLN, lamina propria from small intestine (SI), and large intestine (LI; Lin⁻CD90.2⁺CD127⁺GATA3⁺, pregated on CD45⁺ cells). (B) The number of ILC2s in A were shown (n = 7 per group). (C) Representative flow cytometric analysis of CD69 expression on tissue ILC2s from A. (D) Flow analysis of ICAM-1 expression on the indicated mouse ILC subsets, including spleen NK (CD45⁺Lin⁻NK1.1⁺NKP46⁺), ILC1 (CD45⁺Lin⁻NK1.1⁺NKP46⁺), ILC2 (CD45⁺Lin⁻CD127⁺KLRG1⁺), and ILC3 (CD45⁺Lin⁻CD127⁺Rorγt⁺) in mLN. Mean fluorescence intensity (MFI) levels were shown (n = 6). (E) Frequencies of CD45⁺Lin⁻NK1.1⁺NKP46⁺ NK cells from spleen and CD45⁺Lin⁻NK1.1⁺NKP46⁺ ILC1s from LI in WT and ICAM-1^−/− mice were determined by flow cytometry (n = 6 per group). (F and G) Frequencies of CD45⁺Lin⁻ CD127⁺Rorγt⁺ ILC3s from mLN (F) and LI (G) of WT and ICAM-1^−/− mice were determined by flow cytometry (n = 6 per group). Data are representative of two independent experiments. Error bars show mean ± SEM; **, P < 0.01; ***, P < 0.001 by unpaired Student's t test. ns, not significant. Numbers within flow plots indicate the percentages of cells gated, and arrows from outlined areas at top indicate gated cells analyzed below.