Sarcomere phosphorylation response to bolus injection of DN and OM. Analysis of sarcomere phosphorylation 1 min after infusion of either vehicle control (DMSO), DN at 3 mg/kg (DN Low), DN at 6 mg/kg (DN High), OM at 0.75 mg/kg (OM Low), or OM at 1.5 mg/kg (OM High). (A–E) Representative (A) Coomassie blue– and (B) ProQ Diamond–stained gels with annotations indicating the molecular weights of the ladder and the locations of cMyBPC, cTnI, and myosin’s RLC. ProQ Diamond signals normalized to Coomassie blue signal for (C) cMyBPC, (D) cTnI, and (E) RLC. N = 4 hearts/group. No comparison was statistically significant and all P > 0.05 as determined by ANOVA. Exact P values are as follows: (C) P = 0.52; (D) P = 0.54; (E) P = 0.53. Source data are available for this figure: SourceData F7.