LKB1-dependent RGC migration is restricted to particular RGC classes. (A and B) Representative images (A) and quantification (B) of additional RGC subtypes in controls and in LKB1^RET mice at P8 (anti-Tusc5, T5-type, and F-RGCs; Spp1/SMI32 and αRGCs; and anti-SATB2, ON-OFF DSRGCs, and F-RGCs in the first postnatal week). Larger RGC categories that contain F-RGC types were reduced in LKB1 mutants, including those marked by SATB2 and TUSC5, while non-F-RGC type (Spp1/SMI32 and αRGCs) remained unaltered at P8. (C) Reanalysis of an E16 retina single-cell RNA-seq database generated by Shekhar et al. (2022) using the Broad Institute Single Cell Portal (Tarhan et al., 2023). Clusters that formed transcriptionally defined RGC types were used to compare LKB1 expression levels between different RGC classes. Lkb1 (STK11) expression levels were significantly elevated in F-RGCs relative to all other RGCs and relative to ooDSRGCs and αRGCs types specifically. *P < 0.05, **P < 0.01, ***P < 0.001, nonparametric Mann–Whitney rank-sum U test.