Conductances between pairs of cells expressing the p.G40S and p.R244P mutants, alone or in combination. (A) Cell pairs expressing the noted mutant or combination of mutants, fail to induce coupling significantly different than that seen in pairs of cells expressing vector alone. (B) Cells expressing the p.G40S, p.R244P, or both of the p.G40S and the p.R244P mutants, paired heterotopically with Cx43 WT fail to induce coupling significantly different than that seen when cells expressing vector alone are paired heterotypically with Cx43 WT. Mean values indicated in red with SEM whiskers. ANOVA with Holm–Šidák post-test, all P values are versus homotypic Cx47 WT (A) or heterotypic Cx47 WT/Cx43 WT (B). Number of cell pairs per configuration from left to right along the abscissae: 13, 15, 12, 10, and 17 (A); 13, 9, 14, 14, and 10 (B). IE = pIRES-EGFP (empty vector). (C) Representative current traces for channels recorded from the indicated pairings. Both cells of a pair were voltage clamped at the same voltage, then cell 1 (expressing the second variant in the configuration) was stepped in 20 mV increments from V_j = −100 to V_j = +100 mV, and junctional currents (I_j) were recorded from cell 2 (expressing the first variant in the configuration). Note that the polarity of I_j is opposite that of V_j.