Cx47 WT and variant simulation RMSDs across time relative to the first frame of simulation. (A–F) Simulation for three replicates (100 ns, blue; 160 ns, red; and 500 ns, green) of WT (A and D), p.G40S (B and E), and p.R244P (C and F). (A–C) include the relatively long, intrinsically disordered cytoplasmic loops and cytoplasmic termini, whereas D–F focus on the transmembrane and extracellular residues, which comprise the membrane channel.