Adipocyte-specific KO of Trx2 results in hyperglycemia and insulin resistance. (A) Representative immunoblot analysis of Trx2 protein in WAT and BAT of Trx2^ADKO mice relative to the WT controls. Trx2 protein levels were quantified and presented as fold changes by taking WT as 1.0. n = 3 male mice. Female mice show similar Trx2 deletion. (B) Representative hematoxylin and eosin images of eWAT and ingWAT from 14-wk-old WT and Trx2^ADKO mice (n = 6 male). Diameters of adipocyte in eWAT and ingWAT were quantified. Female mice show similar phenotype in adipocyte size. Scale bars, 100 µm. (C–F) Food intake, oxygen consumption corrected for lean mass, energy expenditure corrected for lean mass, and RER of 14-wk-old WT and Trx2^ADKO mice (n = 6). (G and H) Fasting blood glucose and serum insulin levels over time in WT and Trx2^ADKO mice (n = 6). (I and J) GTT and ITT in 14-wk-old male WT and Trx2^ADKO mice (n = 8). AUC 120 min was calculated. Quantitative data are presented as mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001 versus the corresponding value for WT. Significance was analyzed by two-tailed Student’s t test. ns, not significant.