Trx2 targeting strategy and adipocyte-specific deletion. (A) Schematic diagram of adipocyte-specific Trx2 KO by the loxP-Cre system. (B) Genotyping results of Trx2^lox/lox (WT) and Trx2^ADKO (Trx2^lox/lox;Adipo-Cre; KO) mice. Note that WT mice did not contain Cre. (C) Representative Western blot analysis of Trx2 expression in eWAT, interscapular BAT (iBAT), and pancreas from WT and KO mice. β-Actin was used as a loading control. TK, thymidine kinase.