Single-cell transcriptome of SLE-prone B cells identifies several clusters. (A) Gating strategy for sorting 90% IgD⁻ activated and 10% IgD⁺ naïve B cells for scRNAseq analysis. Panel A is duplicated in Fig. 6. (B) UMAP depicting cell cycle status of clusters determined based on average gene expression of gene sets annotated for each cell cycle state. (C) Dot plot depicting some major markers used in the annotation of the scRNAseq of B cell clusters, where dot color indicates the average expression of the gene within that cluster and the size of the dot indicates the percentage of the cluster expressing that gene. LZ, light zone; DZ, dark zone.