IRF7 controls autoimmune GC and AFC responses by functioning in hematopoietic cells. (A) The schematic of competition chimeras in which allotype-marked BM from FcγRIIB^−/− (CD45.1⁺CD45.2⁺) and FcγRIIB^−/−IRF7^−/− (CD45.2⁺) mice was transferred into lethally irradiated CD45.1⁺ hosts. (B) The bars and flow cytometry plot show BM reconstitution of FcγRIIB^−/− (CD45.1⁺CD45.2⁺) and FcγRIIB^−/−IRF7^−/− (CD45.2⁺) donor cells. (C and D) Flow cytometry analysis of (C) the frequency and (D) gating strategy of CD38⁺IgD⁺ FO B cells of total B220⁺CD19⁺ splenic B cells. (E–H) Flow cytometry analysis of (E) the frequency and (F) gating strategy of IgD⁻CD11b⁺CD11c⁺ ABCs of B220⁺CD19⁺ total splenic B cells and (G) the frequency and (H) gating strategy of B220⁺GL-7⁺CD95⁺ GC B cells of B220⁺CD19⁺ total splenic B cells. (I and J) Flow cytometry analysis of (I) the frequency and (J) gating strategy of CD138⁺TACI⁺ splenic and BM PCs of IgD⁻ B cells (shown in Fig. 1 H). (K–M) Flow cytometry analysis of the frequencies of splenic (K) CD4⁺ Teffs and (L) Tfh of total CD4⁺ cells and (M) the frequencies of various myeloid cells of total myeloid populations. Each symbol represents an individual mouse (n = 10–14 mice per group), and data are presented as means ± SEM. Data in each panel represent three experiments. P values were calculated via an unpaired Student’s t test (not significant, NS, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001). cDCs, conventional DCs; moDCs, monocyte-derived DCs.