Reduced autoreactive AFC and increased pDC numbers in FcγRIIB ^−/− YaaIRF7 ^−/− mice. (A and B) The number of dsDNA-, nucleosome-, and SmRNP-specific splenic (A) and BM (B) AFCs was quantified by ELISpot in 3-mo-old FcγRIIB^−/− Yaa and FcγRIIB^−/− YaaIRF7^−/− mice. (C) Percentage and number of myeloid cells in these mice. (D) Gating strategy of B220⁺CD317⁺ (PDCA-1) pDCs. Each symbol represents an individual mouse, and data are presented as means ± SEM. P values were calculated via an unpaired Student’s t test or Mann–Whitney test (ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001).