E93 regulates Draper and Crq to facilitate NC death. (A and B) Eip93F^RNAi exhibits reduced levels of Crq and Drpr transcripts; tub84b is the loading control. Quantification of the transcripts in the Eip93F^RNAi-overexpressing egg chambers with respect to the control. (C) Eip93F physically interacts with Crq promoter in yeast. Overnight grown cultures of Y1H Gold yeast strain containing bait (pABAi-CrqPromoter^2.2KB) and prey (pGADT7-E93) constructs that were diluted to an OD600 of 1. 3× serial dilutions (10⁻¹, 10⁻², 10⁻³) were spotted on double dropout medium supplemented without and with aureobasidin inhibitor (300 ng/ml). Pictures were taken after 5 days of incubation at 30°C. (D′–G″) Representative image of stage 14 egg chambers of control (D′ and D″), Eip9F3^RNAi; UAS mCD8GFP (E′ and E″), UAS Drpr; UAS Eip93F^RNAi (F′ and F″), UAS Drpr; UAS mCD8GFP (G′ and G″). Arm is in green, DAPI is in red, and GFP is in cyan. Yellow arrowheads mark the persisting NC nuclei. (H) Quantification of persisting NC nuclei in stage 14 egg chambers of indicated genotypes. n stands for the number of egg chambers analyzed and the mean number of PN observed for each genotype. (I) Number of PN in stage 14 egg chamber as categorized into bins of 0 PN, 1–3 PN, 4–6 PN, 7–10 PN, 11–15 PN, and the percentage of stage 14 egg chambers in each bin was calculated. Error bars represent the SEM. ****P ≤ 0.0001, **P ≤ 0.001, *P ≤ 0.05 represent the level of significance. Source data are available for this figure: SourceData F7.