Figure 5.
Ecdysone pathway regulates the expression of Draper and Croquemort to facilitate NC removal. (A and B) EcRB1DN exhibits reduced Crq and Drpr expression; tub84b is the loading control. Quantification of the transcripts in the EcRDN-overexpressing egg chambers with respect to the control. (C1–D4) Ecdysone modulates Drpr expression in late-stage 12 egg chambers. Draper is in magenta (C3 and D3) or LUT (C4 and D4), and DAPI is in cyan (C1 and D1). The AFC membrane is marked by mCD8GFP in green (C2 and D2). AFCs are encircled by a white line (C1–C3 and D1–D3) and by a red line (in C4 and D4). (E) Quantification of Draper in control and EcRB1DN. Draper staining in the AFCs is normalized to the background staining of posterior follicle cells. Please note the Draper is lower in EcRDN-overexpressing egg chambers. (F′–J″) Overexpression of Crq and Drpr individually rescues NC clearance defect exhibited by EcRB1DN-overexpressing egg chambers. Yellow arrowheads mark PN. Representative image of stage 14 egg chambers of the indicated genotypes in control (F′ and F″), EcRB1DN; UAS mCD8GFP (G′, G″, I′, and I″), EcRB1DN; UAS CrqHA (H′ and H″), and EcRB1DN; UAS Drpr (J′ and J″). Egg chambers were stained for Arm or F-actin in green, and DAPI is in red. (K) Quantification of persisting NC nuclei in stage 14 egg chambers of indicated genotypes. The mean number of PN for each genotype is represented. (L) Number of PN in stage 14 egg chamber of indicated genotypes as categorized into bins of 0 PN, 1–3 PN, 4–6 PN, 7–10 PN, 11–15 PN, and the percentage of stage 14 egg chambers in each bin is depicted. Error bars represent the SEM. ****P ≤ 0.0001, **P ≤ 0.001 represent the level of significance. Source data are available for this figure: SourceData F5.

Ecdysone pathway regulates the expression of Draper and Croquemort to facilitate NC removal. (A and B) EcRB1DN exhibits reduced Crq and Drpr expression; tub84b is the loading control. Quantification of the transcripts in the EcRDN-overexpressing egg chambers with respect to the control. (C1–D4) Ecdysone modulates Drpr expression in late-stage 12 egg chambers. Draper is in magenta (C3 and D3) or LUT (C4 and D4), and DAPI is in cyan (C1 and D1). The AFC membrane is marked by mCD8GFP in green (C2 and D2). AFCs are encircled by a white line (C1–C3 and D1–D3) and by a red line (in C4 and D4). (E) Quantification of Draper in control and EcRB1DN. Draper staining in the AFCs is normalized to the background staining of posterior follicle cells. Please note the Draper is lower in EcRDN-overexpressing egg chambers. (F′–J″) Overexpression of Crq and Drpr individually rescues NC clearance defect exhibited by EcRB1DN-overexpressing egg chambers. Yellow arrowheads mark PN. Representative image of stage 14 egg chambers of the indicated genotypes in control (F′ and F″), EcRB1DN; UAS mCD8GFP (G′, G″, I′, and I″), EcRB1DN; UAS CrqHA (H′ and H″), and EcRB1DN; UAS Drpr (J′ and J″). Egg chambers were stained for Arm or F-actin in green, and DAPI is in red. (K) Quantification of persisting NC nuclei in stage 14 egg chambers of indicated genotypes. The mean number of PN for each genotype is represented. (L) Number of PN in stage 14 egg chamber of indicated genotypes as categorized into bins of 0 PN, 1–3 PN, 4–6 PN, 7–10 PN, 11–15 PN, and the percentage of stage 14 egg chambers in each bin is depicted. Error bars represent the SEM. ****P ≤ 0.0001, **P ≤ 0.001 represent the level of significance. Source data are available for this figure: SourceData F5.

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