Ecdysone regulates Eip93F and JNK signaling in the AFCs. (A–E) Representative image of stage 14 egg chambers of indicated genotypes. Egg chambers are stained with DAPI in red. A yellow arrowhead marks the PN in the anterior end. (F and G) Downregulation of phagocytic receptors by RNAi using GR1-Gal4. Quantification of persisting NC nuclei in stage 14 egg chambers of indicated genotypes. n indicates the number of egg chambers analyzed (F). The number of PN in stage 14 egg chamber was categorized into bins (G). The percentage of stage 14 egg chambers in each bin was calculated. n indicates the number of egg chambers analyzed. Please note that these phagocytic receptors probably do not have a significant role in NC clearance in late oogenesis. (H) Number of PN in stage 14 egg chamber of indicated genotypes was categorized into bins. The percentage of stage 14 egg chambers in each bin was calculated. (I¹–J³) Ecdysone modulates the JNK pathway. The status of puc-lacz is in green in control (I¹–I³) and EcRB1^DN (J¹–J³). DAPI is in red. (K) Quantification of normalized puc-lacz expression in control and EcRB1^DN. (L¹and L²) EcRB1^DN exhibits reduced levels of Eip93F transcripts; tub84b is the loading control. Quantification of the Eip93F transcripts in the EcRB1^DN-overexpressing egg chambers with respect to the control. (M) Number of PN in stage 14 egg chamber of indicated genotypes was categorized into bins, and the percentage of stage 14 egg chambers in each bin is listed. (N) Number of PN in stage 14 egg chamber of indicated genotypes was categorized into bins, and the percentage of stage 14 egg chambers in each bin is listed. n indicates the number of egg chambers analyzed. Error bars represent the SEM. ****P ≤ 0.0001, **P ≤ 0.001, ns = nonsignificant represent the level of significance.