Figure 4.

Transition from epithelial to nonprofessional phagocyte helps to remove NCs. (A–C) Expression pattern of crq-Gal4 during different stages of oogenesis: stage 10 (A), stage late 11 (B), and stage 13 (C). Note: UAS mCD8GFP is driven by crq-Gal4 (in magenta). DAPI marks the nuclei in white. (D′–H″) Representative images of stage 14 egg chambers of indicated genotypes in control (D′ and D″), SrpRNAi (E′ and E″), DrprRNAi (F′ and F″), CrqRNAi (G′ and G″), and CrqRNAi_2 (H′ and H″). Yellow arrowheads mark persisting NC nuclei. (I) Quantification of persisting NC nuclei in stage 14 egg chambers of indicated genotypes. (I)n indicates the number of egg chambers analyzed. The mean of PN of indicated genotypes is mentioned in the graph. (J) Quantification of the number of PN for indicated genotypes. Stage 14 egg chambers were categorized into three groups: 0 PN (without defect), 1–2 PN (mild defect), and PN ≥ 3 (defective egg chambers), and the percentage of stage 14 egg chambers in each bin was calculated. n indicates the number of egg chambers analyzed. (K) Quantification of persisting NC nuclei in stage 14 egg chambers of indicated genotypes. The mean of PN of indicated genotypes is mentioned in the graph. Error bars represent the SEM. ****P ≤ 0.0001, ***P ≤ 0.001, **P ≤ 0.001 represent the level of significance.

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