Rora expression regulates ILC3 development and function. (A) RORγt expression levels in Lineage⁻CD127⁺RORγt⁺ ILC3s (n = 3 mice). (B) Representative flow cytometry plots of CD45⁺Lineage⁻IL-25R⁺ ILC2s in the SI. (C) Quantification of ILC2 numbers in the SI (n = 4–5 mice). (D) UMAP of scRNA-seq of ILC3s in the cecum during intestinal fibrosis, revealing three distinct clusters: DN ILC3s, NCR⁺ ILC3s, and CCR6⁺ ILC3s (reanalyzed from Lo et al. [2019a]). (E) UMAP of scRNA-seq depicting ILC3 subsets in RORα^sg/sg mice and control littermates. (F) Heatmap showing the frequency of ILC3 subsets differing between RORα^sg/sg mice and control littermates during intestinal fibrosis. WT mice refer to Rora^fl/fl littermate controls. Data from A and C are representative of two independent experiments. Statistical significance was determined using an unpaired two-tailed t test (*P < 0.05; ns, not significant).