Figure 2.

Il17rb-eGFP marks all ILC2 irrespective of their distinct, tissue-specific coreceptor expression programs. (A) Representative flow cytometry plots showing Il17rb-eGFP expression in ILCs from the fetal gut at E18.5. (B) Flow cytometry plots depicting Il17rb-eGFP expression in Lineage cells from the fetal gut. (C) Flow cytometry plots of live CD45+LineageIl17rb-eGFP+ cells in the SI and BM of 12-wk-old adult mice. (D) Representative flow cytometry plots of ILC2s in adult Peyer’s patches. (E and F) Flow cytometry plots of ILC2s in the lungs of Il17rb-CreERT2-eGFP mice. (G) Flow cytometry plots of ILC2s in the adult skin, accompanied by representative histograms showing Il17rb-eGFP and IL-25R expression. (H) Flow cytometry plots of ILC2s in the muscle with a representative histogram of Il17rb-eGFP expression. Data from all panels are representative of three or more independent experiments.

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