Il17rb expression in the BM and during IL-33–induced type 2 inflammation. (A) Representative flow cytometry plots showing Il17rb expression in CLPs and CHILPs (immunophenotype: CD45+Lineage−Sca1−/intCD25−). (B) Representative flow cytometry plots of Il17rb expression in CD45+Lineage−Sca1highCD25+ ILC2s. (C) Representative histogram of eGFP expression by LSK (Lineage−Sca-1+C-kit+) versus BM ILC2s (Lineage−CD127+Sca-1+CD25+) in Il17rb-CreERT2-eGFP and C57BL/6 mice. (D) Representative flow cytometry plots showing Il17rb expression in eosinophil progenitors. (E) Representative histogram depicting Il17rb expression in lung ILC2s (Lineage−CD127+ST2+KLRG1+) during IL-33–induced inflammation (gray: C57BL/6; red: Il17rb-CreERT2-eGFP). (F) Representative flow cytometry plots showing Il17rb expression in live CD45+Lineage−CD127+ST2+ ILC2s. (G) Representative histogram comparing Il17rb expression in live CD45+CD3+ T cells and Lineage−CD127+ST2+ ILC2s in the lungs. (H)Il17rb expression in CD45+CD11c−CD11b+SiglecF+ eosinophils during IL-33–induced airway inflammation. Data are representative of two to three independent experiments. CLPs, common lymphoid progenitors; CHILPs, common helper innate lymphoid progenitors.
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