Il17rb expression in the thymus. (A) Representative flow cytometry plots showing Il17rb-eGFP expression within live CD45⁺Lineage⁻CD127⁺ cells. (B) Representative histogram depicting CD25 and Sca-1 expression on live CD45⁺Lineage⁻CD127⁺Il17rb⁺ cells in the thymus. (C) Representative flow cytometry plots of Il17rb expression within CD45⁺CD1d⁺ NKT cells in the thymus. (D) Representative gating strategy for DN progenitors in the thymus (DN1 cells: CD45⁺CD4⁻CD8⁻CD44⁺CD25⁻, DN2 cells: CD45⁺CD4⁻CD8⁻CD44⁺CD25⁺, DN3 cells: CD45⁺CD4⁻CD8⁻CD44^−/lowCD25⁺, DN4 cells: CD45⁺CD4⁻CD8⁻CD44^−/lowCD25⁻). (E)Il17rb expression across DN1, DN2, DN3, and DN4 subsets (n = 3 mice). (F)Il17rb expression levels in Lineage⁻Il17rb⁺ cells isolated from the SI, PP, and thymus (n = 3 mice). Data are representative of two independent experiments and are presented as the mean ± SEM. Statistical significance in F was determined by a one-way ANOVA with Tukey’s multiple comparison test (***P < 0.001; ns, not significant). PP, Peyer’s patches.