Yeast two-hybrid analysis reveals interaction of Pex11 with itself and of Pex25 with itself. Analysis of the interaction of different H. polymorpha proteins with Pex11 and Pex25, using yeast two-hybrid assays. Genes were fused to the LEXA binding domain (LexA-BD) in vector pBTM116 and a VP16 activation domain (Vp16-AD) in vector pVP16. The resulting plasmids were cotransformed into S. cerevisiae L-40. As controls, empty pVP16 or pBTM116 was used for transformation. Pex3 Pex19 interaction was added as positive control. Three independent transformants were tested using a β-galactosidase filter lift assay. Colonies were stained for 8 h.