Figure 2. Quantification of peroxisome numbers in various H. polymorpha strains. Cells were grown on glycerol/methanol mixtures for 16 h. The number of peroxisomes in nonbudding cells was counted from randomly taken CLSM images. For each sample peroxisomes were counted from 2 × 100 cells from two independent experiments. The frequency distributions of cells with number of peroxisomes per cell are shown. Bars represent the SEM. (A) Frequency distributions of WT, pex25, pex11C, and pex25 pex11C cells. (B) Distributions in WT, pex11, pex11 pex11C, and pex11 pex25. In pex11 pex25 peroxisomes could not be detected using the fluorescent matrix marker protein (DsRed-SKL).
Figure 2.

Quantification of peroxisome numbers in various H. polymorpha strains. Cells were grown on glycerol/methanol mixtures for 16 h. The number of peroxisomes in nonbudding cells was counted from randomly taken CLSM images. For each sample peroxisomes were counted from 2 × 100 cells from two independent experiments. The frequency distributions of cells with number of peroxisomes per cell are shown. Bars represent the SEM. (A) Frequency distributions of WT, pex25, pex11C, and pex25 pex11C cells. (B) Distributions in WT, pex11, pex11 pex11C, and pex11 pex25. In pex11 pex25 peroxisomes could not be detected using the fluorescent matrix marker protein (DsRed-SKL).

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