PPARγ2 localization in calreticulin-deficient G45 and calreticulin-expressing CGR8 ES cells. At D20 of differentiation, the cells were labeled for PPARγ2 and propidium iodide (PI) to identify the nuclei as described in Materials and methods. (A) In the calreticulin-deficient cells, PPARγ2 (green) colocalized with propidium iodide (red). (B) In the calreticulin-containing cells, labeling for PPARγ2 was barely detectable and perinuclear. Bar, 25 μm.