Group-specific TF core regulatory circuitries are recapitulated in primary tumors and coopted in NSCs. (a) Assigning the primary GBM samples by H3K27ac signal at the SEs of group 1 gene signature (group 1–specific TFs, n = 14; target genes, n = 125) and group 2 gene signature (group 2–specific TFs, n = 18; target genes, n = 171) by k-means. Assignment was reconfirmed by using gene expression of the two gene signatures (group 1–like primary GBMs = 38; group 2–like primary GBMs = 5). The figure shows the principal components of the GSCs and GBMs for log2 (FPKM) of the above set of genes along with groups to which they were assigned. (b) The same as in Fig. 4 b but for primary GBMs (group 1–like primary GBMs = 6; group 2–like primary GBMs = 1). (c) The same as in Fig. 4 c with gene expression of the sample on the right. (d) Similarity of single cells of five primary GBMs to group 1 and group 2 GSCs using the gene signatures used for panel a. Left: The average gene expression of the group 1 gene signature and group 2 gene signature in every single sample. Right: The median Spearman rank-correlation with P < 0.01 ≥75% of group 1 and group 2 GSCs.