Active chromatin landscape of GBM and GSCs. (a) ChIP-seq H3K27 acetylation profile surrounding the SOX2 locus across GBM. (b) DNA methylation levels (Illumina EPIC arrays) surrounding the SOX2 locus. (c) t-SNE analysis of the top 10,000 variant H3K27ac loci of GBM primary tumors, GSCs, medulloblastoma primary tumors, ependymoma primary tumors, Roadmap consortium samples, and ENCODE consortium samples (Kundaje et al., 2015). n = 250 samples. (d) t-SNE analysis of the top 10,000 variant CpG loci across study GBM, GSCs, and DNA methylation brain tumor classification reference samples (Capper et al., 2018). HSC, hematopoietic stem cell; iPSC, induced pluripotent stem cell; AM, placental amnion; EPD, ependymoma; MB, medulloblastoma.