Figure 7.
The overexpression of DRP1 increases peripheral junctions in PINK1-depleted cells and suppresses the ER-phagy defect. (A) siCtrl and siPINK1-treated cells containing Akita-sfGFP were transfected with mCherry-DRP1 or mCherry-DRP1 D-octadecapeptide (554–571 aa). Top, representative images for the data graphed below. (B) Quantification of Akita-sfGFP puncta in LAMP1-mCherry structures in siCtrl and siPINK1-treated cells that were untreated or incubated with Baf (3.5 h) in the absence and presence of mCherry-DRP1. The control for each condition was set to 1.0. Error bars represent SEM, n = 3 independent experiments. The results were quantified from 100 to 195 cells in A, left, 67–149 cells in A, middle, 33–47 cells in A, right, 60–97 cells in B. NS: not significant (P ≥ 0.05), * (P < 0.05), **(P < 0.01), *** (P < 0.001), Student’s unpaired t test.

The overexpression of DRP1 increases peripheral junctions in PINK1-depleted cells and suppresses the ER-phagy defect. (A) siCtrl and siPINK1-treated cells containing Akita-sfGFP were transfected with mCherry-DRP1 or mCherry-DRP1 D-octadecapeptide (554–571 aa). Top, representative images for the data graphed below. (B) Quantification of Akita-sfGFP puncta in LAMP1-mCherry structures in siCtrl and siPINK1-treated cells that were untreated or incubated with Baf (3.5 h) in the absence and presence of mCherry-DRP1. The control for each condition was set to 1.0. Error bars represent SEM, n = 3 independent experiments. The results were quantified from 100 to 195 cells in A, left, 67–149 cells in A, middle, 33–47 cells in A, right, 60–97 cells in B. NS: not significant (P ≥ 0.05), * (P < 0.05), **(P < 0.01), *** (P < 0.001), Student’s unpaired t test.

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