Related to Figs. 5, 6, and 7. mCherry-DRP1 suppresses the ER morphology defect in siPINK1-treated cells. (A) Left, western blot analysis was performed using cell lysates depleted of Parkin. Samples were blotted for Parkin and GAPDH. Right, representative images of GFP-Mito7 in Ctrl and siParkin-treated cells. (B) Same as Fig. 6 D middle, only the data was not normalized. (C) siCtrl and siPINK1-treated cells containing Akita-sfGFP were transfected with mCherry-Mff, or LNPK-mCherry. Left, representative images for the data graphed on the right. The remaining images are shown in Fig. 7 A. (D) Left, siCtrl and siPINK1-treated cells were transfected with mCherry-DRP1 (lane 3) and mCherry-Mff (lane 4) and blotted for the expression of the mCherry fusion proteins. Lane 2 does not contain plasmid. Right, Cells were transfected with mTurquoise2-DRP1 and mCherry-Mff. Arrowheads mark colocalizing puncta on mitochondrial membranes. (E) The percentage of cells with abnormal ER was quantitated as described in the methods. Error bars in B, C, and E represent SEM, n = 3 independent experiments. The results were quantified from 78 to 82 cells in B, 100–195 cells in C, top, 33–38 cells in C, bottom, and 78–154 cells in E. NS: not significant (P ≥ 0.05), * (P < 0.05), *** (P < 0.001), Student’s unpaired t test. Source data are available for this figure: SourceData FS12.