Related to Figs. 5 and 6. PINK1-containing puncta are in close proximity to ER junctions. (A) Left, western blot analysis was performed using lysates from control and PINK1-depleted cells. The precursor (p) and mature (m) forms of PINK1 are marked in siCtrl samples. Right, KLHL12 was blotted in Ctrl and siPINK1-treated cells. GAPDH was used as a loading control. (B) Representative control images of YFP-RTN3L puncta in LAMP1-mCherry structures in siCtrl and siPINK1-treated cells for the graph shown in Fig. 5 A. (C) Representative control images of LNPK-GFP puncta in LAMP1-mCherry structures in siPINK1-treated cells for the graph shown in Fig. 5 B. (D) A representative image of a cell transfected with PINK1-YFP and mCherry-RTN3L. Arrowheads mark PINK1-YFP puncta near junctions. (E) Representative PINK1-YFP (used in Fig. 5 D) and mCherry-PINK1 (used in Fig. 5 F) images. The inset in the PINK1-YFP image was used to make Video 1 and the stills in Fig. 5 D. This area was chosen as it is largely devoid of mitochondria. (F) Left, western blot showing KEAP1 levels in control and siRNA-treated cells. Right, qPCR was performed with cells treated with siKEAP1. (G) Compressed Z stacks of Ctrl and siPINK1-treated cells that were transfected with mCherry-KDEL. A total of 14 slices were compressed for Ctrl cells, and 22 slices for siPINK1-treated cells. Source data are available for this figure: SourceData FS11.