Related to Fig. 2 . SopF does not inhibit RTN3L–SEC24C-mediated ER-phagy. (A) Left, a western blot showing the depletion of FIP200 in siFIP200-treated cells. GAPDH was used as a loading control. Right, quantitation of LNPK-GFP puncta in siCtrl and siFIP200-treated cells. Representative images are shown on the left. (B) Left, a representative image of a control cell and a siFIP200-treated cell showing the accumulation of a large Akita puncta (see arrowhead). Right, the percent cells with large Akita puncta in control and FIP200-depleted cells. (C) Cells were transfected with Akita-sfGFP or Akita-sfGFP and mCherry-SopF. Representative images are shown on the left and the percent cells with large Akita puncta is graphed on the right. (D) A representative image for the data quantitated in Fig. 2 C. Arrowheads mark Akita-sfGFP puncta that colocalize with LNPK-mCherry puncta. (E) Representative images for the data graphed in Fig. 2 D. Arrows mark large Akita puncta. Error bars in A–C represent SEM, n = 3 independent experiments. The results were quantified from 61 to 64 cells in A, 56–104 cells in B, and 92–95 cells in C. NS: not significant (P ≥ 0.05), **(P < 0.01), Student’s unpaired t test. Source data are available for this figure: SourceData FS6.