Related to Fig. 1 . LIRs 1–5 are not needed for the colocalization of SEC24C puncta with RTN3L puncta. (A) Left, a western blot showing that the expression of RTN3L is the same in cells stably expressing mCherry–RTN3L or mCherry–RTN3LΔ6LIR. Right, cells stably expressing mCherry–RTN3L or mCherry–RTN3LΔ6LIR were treated with Torin 2 or MRT68921 for 3.5 h and imaged. (B) Diagram showing the LIR mutations in mCherry-RTN3L. The 6LIRs are shown as black bars and the mutated amino acids are in red. (C) Representative images for the constructs shown in B. The percent SEC24C puncta colocalizing with RTN3L puncta were quantified for the constructs shown in B. Cells were treated for 3.5 h with Torin2. Error bars in C represent SEM, n = 3 independent experiments. The results were quantified from 60 to 66 cells. NS: not significant (P ≥ 0.05), Student’s unpaired t test. Source data are available for this figure: SourceData FS4.