MTMR 2/3/4/7 regulate cellular PI3P contents. T47D cells expressing SCR or shRNA targeting MTMR 2, 3, 4 or 7, followed by 1 µg/ml puromycin selection, were overexpressed with GFP-2xFYVE. Cells were incubated with CellMask for 1 h at 37°C incubator, fixed with 4% PFA, and imaged by confocal microscopy. A selected region (the white square) is shown at a higher magnification. Arrowheads indicate the PM staining of GFP-2xFYVE. Scale bar—10 μm. SCR, scrambled shRNA.