Characterization of colonic and systemic inflammation in W/W mice. (A) Colitis score based on immunohistochemical analysis of 12-wk-old (wo) wild-type (+/+) mice or Rag1^w/w (W/W) mice split by sex (female, F; male, M; n = 5–10/group). Bars show the mean with SEM. Statistical analysis was performed using a Mann–Whitney test. (B) Gene expression from total large intestine lysate (n = 5/group). Statistical analysis was performed using a Mann–Whitney test. (C and D) Representative plot (C) and frequency (D) of myeloid cells isolated from the LP of 12wo +/+ mice or W/W mice (n = 5/group). Statistical analysis was performed using a Mann–Whitney test. (E) Representative plot and frequency of naïve (CD62L⁺CD44⁻) and effector (CD62L⁻CD44⁺) CD4⁺ T cells isolated from MLN of 12wo +/+ mice (n = 10) or W/W mice (n = 7). Statistical analysis was performed using a Mann–Whitney test. (F) Representative plot and frequency of integrin α4β7⁺ cells on effector (CD62L⁻CD44⁺) CD4⁺ T cells isolated from MLN of 12wo +/+ mice (n = 10) or W/W mice (n = 7). Statistical analysis was performed using a Mann–Whitney test. (G) Serum cytokine levels from 12wo +/+ mice (n = 6), or 5 and 12 wo W/W mice (n = 3-3-63-66/group). Data are from more than three independent experiments. Groups were compared using a Kruskal–Wallis test with Dunn’s multiple comparisons correction. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.