Gating strategies for flow cytometry. (A) Gating strategy for DN, DP, CD8⁺, CD4⁺ (SP) T cells, and Foxp3⁺ cells. (B) Gating strategy for mTEC^hi, mTEC^lo, and cTEC populations. (C) Gating strategy for splenic Tfh, Tfr, Tconv, and Tregs. (D) Gating strategy for lymph node Tfh, Tfr, Tconv, and Tregs. (E) Gating strategy for activated natural killer (NK) cells. (F) Gating strategy for CD45⁺ cells in the salivary glands.