Aire is dispensable in oral tolerance mediated by RORγt-lineage APCs. (A) Quantification of Aire⁺ MHCII⁺ cells from the thymus (top) and LNs (bottom) in Aire^ΔRORγt, Aire^ΔVav1, or WT mice (n = 6 from each group). (B) Oral tolerance experimental setup is the same as Fig. 1 A. Flow cytometry quantification of OT-II T cells from Aire^ΔRORγt, Aire^ΔVav1, or WT mouse mLN after 2 days of OVA gavage (n = 6 for WT, n = 4 for Aire^ΔRORγt, n = 2 for Aire^ΔVav1). Data are representative of three independent experiments. Error bars: mean ± SEM; statistics were calculated by an unpaired two-sided t test (A) and one-way ANOVA with Tukey’s multiple comparisons test (B); ****P < 0.0001.