Reconciling the diversity of RORγt-lineage APC populations. (A–D) Spleen, mLN, and other LNs (cervical, inguinal, axillary, brachial, and para-aortic) from adult RORγt-lineage mice (RORγt^Cre×Rosa26-LSL-tdTmt) were harvested, digested, magnetically depleted of T and B cells, FACS-sorted (tdTmt⁺ MHCII⁺), and subjected to single-cell sequencing. (A) Reduced dimensionality representation of our adult scRNA-seq data with annotated cell clusters. (B) Select gene expression in RORγt-lineage APC subsets. (C) Top: Reduced dimensionality representation of R-eTAC and R-DC subsets. Bottom: Gene expression of Aire and Rorc in those subsets. (D) Differential gene expression heatmap of RORγt-lineage APC subsets. (E) Top: Integrated UMAP of all available RORγt-lineage APC single-cell data, including Wang et al. (2021), Lyu et al. (2022a), Kedmi et al. (2022), Akagbosu et al. (2022), and our 2-wk-old data (left to right). Bottom: Corresponding CellTypist projections onto our adult scRNA-seq data.