Parasympathetic activity does not affect ACC proliferation or correlate with survival. (A) RT-PCR of relative cholinergic muscarinic receptor expression of AH43a and AH45b. (B) MTT assay of AH43a and AH45b cells treated with carbachol for 24 h or control (n = 6 per group). (C) Tumor volumes at week 3 of AH45b PDX grown in SMG, PG, and flank, treated with scopolamine (n = 15) or control (n = 14). (D) Kaplan–Meier survival curves stratified by parasympathetic neuronal gene expression signature IPAS scores. Parasympathetic signatures do not show significant differences in survival between the high and low groups, stratified by the lowest P value. Parasympathetic neuron shows a nonsignificant trend using the log-rank (Mantel–Cox) test. Data are representative of two independent experiments (B) or one experiment (C). P values were determined by one-way ANOVA, Fisher’s LSD test (B), Student’s t test (C), or log-rank (Mantel–Cox) test (D). ns: not significant.