Reduced intratumoral nerve sprouting and no effect of sympathetic ablation in a non-ACC salivary cancer. (A) Large-caliber TH⁺ nerve (dashed circle) lacks any small caliber TH⁺ nerve sprouts in the non-ACC, mucoepidermoid salivary cancer, HTB-41, grown in the mouse SMG. Scale bar: 50 μm. Quantification of SMG intratumoral TH⁺ nerve sprouts for AH43a (n = 5), AH45b (n = 8), and HTB-41 (n = 8). AH43a and AH45b SMG TH⁺ data are derived from Fig. 3 H. (B) Volumes of HTB-41 tumors 3 wk after implantation in the mouse SMG, treated with either sham surgery (n = 9) or sympathectomy (n = 11). (C) RT-PCR of relative β2R expression in AH43a, AH45b, and HTB-41, using β-actin as endogenous control. Data represent one experiment (A–C). P values were determined by one-way ANOVA, Fisher’s LSD test (A), or Student’s t test (B). *P < 0.05; ns: not significant.