Correlation between standard free energies and ΔT _m . (A) Plot of standard free energy of binding versus ΔT_m for E34Q, S24K, E36V, and S24L. (B) Plot of standard free energy of binding versus ΔT_m for E34Q, S24K, E36V, S24L, and Δ11. ΔT_m for WT is 0. Lines represent a linear fitting.