The E34Q mutation in the Tpm-binding site of Lmod2 slightly decreases the binding affinity to Tpm1.1. (A) Representative measurements showing the temperature dependence of the ellipticity at 222 nm measured for unmixed and mixed αTM1a_1–14Zip and Lmod2s1: Lmod2s1[E34Q] (●), αTM1a_1–14Zip (▼), a mixture of Lmod2s1[E34Q] and αTM1a_1–14Zip (■), and a sum of the individual curves for Lmod2s1[E34Q] and αTM1a_1–14Zip (□). (B) The temperature dependence of the ellipticity at 222 nm measured for mixtures of αTM1a_1–14Zip with Lmod2s1[WT] (Δ) and Lmod2s1[E34Q] (■). Each data point represents a 5-s average of the signal, with SEM shown as bars.