Figure 3.
Schematic representation of dual-intein EGFP pLenti containing the nucleotides surrounding the ATM splitting point. In the pLenti encoding for the EGFP N-term half ATM CDS corresponds to nucleotides 3826…3852. In the pLenti encoding for the C-term EGFP, ATM CDS corresponds to nucleotides 3853…3879 and comprises the triplet encoding for Cys1286. nt: nucleotides.